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Table 2 Summary of previous studies on four-dimensional (4D) tracking of spermatozoa using digital in-line holographic microscopy

From: Digital in-line holographic microscopy for label-free identification and tracking of biological cells

Year

Object

Content

Reconstruction method

Axial localization algorithm

Tracking algorithm

References

2012

Human spermatozoon

Swimming patterns of spermatozoa, such as typical, helical, hyper-activated, and hyper-helical patterns

Weighted back-projection method

Local extreme contrast

Linking up the nearest three-dimensional (3D) positions of objects in subsequent holograms

[152]

2022

Human spermatozoon

Swimming motions of human spermatozoa

Angular spectrum method

DarkFocus algorithm

Connecting 3D positions for each object in subsequent holograms

[153]

2022

Human and goat spermatozoa

Comparison of swimming motions of human and goat spermatozoa

Angular spectrum method

DarkFocus algorithm

Connecting 3D positions for each object in subsequent holograms

[154]

2015

Arbacia punctulata (A. punctulata) spermatozoon

Swimming motions of A. punctulata spermatozoa navigating 3D chemoattractant gradients provided by an egg for fertilization

Rayleigh-Sommerfeld back-propagation

Gouy phase anomaly and Sobel filtering

Linking 3D positions into continuous trajectories using a custom-made tracking program written in Java

[155]

2016

Horse spermatozoon

Swimming patterns of horse spermatozoa, such as irregular, linear, planar, helical, ribbon, and hyper-progressive patterns

Weighted back-projection method

Local extreme contrast

Linking up the nearest 3D positions of objects in subsequent holograms

[156]

2021

Mouse spermatozoon

Comparison of swimming motions of a normal mouse and a mouse without tubulin glycosylation

Rayleigh-Sommerfeld back-propagation

Gouy phase anomaly

Connecting 3D positions for each object in subsequent holograms

[157]